American test tube expert:How to successfully reproduce with high sperm fragmentation rate？

During the delivery process,Both men and women play important roles。The uterus that carries eggs and nurtures life in women,And men need to provide high-quality sperm。therefore,No matter how well prepared women are,No matter how well the body is regulated,If she encounters weak sperm,It is still useless。today,American IVF experts will analyze how to solve the fertility problem of high sperm fragmentation rate for you,And successfully obtaining a healthy baby。

Sperm fragmentation rate

according to the statistics,Male factors account for the proportion of infertile couples40%,Among them, male spermDNAHigh fragmentation rate is an undeniable factor。Many people believe that,As long as there are no issues with routine semen testing for males,Everything will be all right.。in fact,Semen routine only judges whether sperm are normal based on some external indicators,We also need some internal testing indicators to evaluate the quality of sperm。

spermDNAFragments refer to the harmful factors that affect sperm formation during the process,Thereby damaging the intact spermDNA,Forming fragmentedDNASmall fragments。Measuring spermDNAThe indicator of fragmentation degree is called spermDNAFragmentation rate,abbreviationDFI,It refers toDNAPercentage of sperm with broken chains among all sperm。Also known as spermDNAIntegrity testing,Because it can reflect spermDNAIntegrity of fragments。

Many studies have shown that,spermDNADamage can lead to male infertility,Reduce the natural pregnancy rate,Increased risk of miscarriage。The success rate of IVF will affect the quality of IVF。

that,spermDNAHow high is the fragmentation rate normal?Usually using spermDNAFragmentation index(DFI)High and low representation:Currently, it is widely believed that:DFI≤15%normal,15%normal

Four major IVF technologies in the United States help men solve infertility problems,Successfully acquiring healthy children

1、PerecollGradient centrifugation sperm washing technology

After going to the United States,American in vitro fertilization experts will useperecollGradient centrifugation sperm washing technology for washing and optimizing semen,Remove components that are detrimental to egg fertilization,Like dead sperm、Inflammatory sperm、Sperm antibodies、High rates of inflammatory cells and sperm fragments,Then observe it under a high-power microscope to see if it is normal、Vibrant and healthy sperm separated。

2、The second-generation IVF technology in the United States

United StatesHRCReproductive experts obtain healthy sperm,Will use second-generation IVF technologyICSI,Single sperm injection technique in the cytoplasm of oocytes,With the help of a micro operating system,Injecting a single sperm into the egg for fertilization。Under high magnification microscopy system,A reproductive doctor will use a very thin glass straw to absorb one side of a mature egg,Use another to absorb healthy sperm,Directly injected into the egg,Make sperm bypass the zona pellucida and plasma membrane of the egg,Direct fertilization。It not only overcomes the disadvantage of low success rate of free fertilization between sperm and egg,And it can effectively combine sperm and egg,Make sperm problems no longer a problem,Laying a solid foundation for the success of test tubes。

Throughout the entire operation process,Advanced medical equipment in the laboratory is a basic requirement,The strict requirements for doctors' professional operations are very high。however,have34Years of American HistoryHRCThe reproductive hospital has mastered the second-generation in vitro fertilization technology proficiently,thereforeICSITechnology has significant advantages。

3、Strictly implement laboratory standards,Five day pouch raising technique

in the U.S.HRCReproductive Hospital,In vitro fertilization experts place fertilized eggs into a special culture medium in a blastocyst incubator for cultivation,To simulate the uterine environment of the human body,Raise the embryo at a certain temperature、humidity、Oxygen content andpHGrowth and development under conditions similar to uterine environment。On the fifth or sixth day,Will form by100A blastocyst composed of multiple cells。

Embryos can clearly differentiate into trophoblast cells(Formation of placenta in the future)And the inner cell group(Developing into a fetus in the future),Its morphological structure is more stable,Stronger vitality,More active。meanwhile,It is also composed of pluripotent cells,Has good developmental potential,Therefore, the implantation ability of blastocysts will be stronger。

in addition,Embryos are more in line with the physiological state of the uterus,After implantation, it can synchronize with the endometrium,More likely to continue growing and developing。More Than This,Due to significant differentiation of blastocyst cells,Complete structure,When extracting peripheral cells from blastocysts for genetic testing,Can ensure that the quality of blastocysts is not affected,Thus ensuring the integrity of the embryo。

4、Third generation IVF technology in the United States

Third generation IVF technology in the United States,Genetic screening before embryo transfer/diagnosis(PGS/PGD)

Before transplantationPGSEmbryonic genetic screening

United StatesHRCReproductive doctors conduct genetic testing by extracting peripheral cells from blastocysts,yes23Comparative analysis of chromosome structure and blastocyst quantity,Filter for duplicates、inversion、translocation、Abnormal situations such as missing items,Then choose a healthy blastocyst。therefore,In this step,Can determine whether the embryo has chromosomal abnormalities。

PGDGenetic diagnosis before embryo transfer

This technology targets chromosomal genes,Diagnose whether the embryo carries genetic defects or gene mutations that may lead to specific diseases,Detecting a single chromosomeDNAFragment elimination of diseases caused by gene defects,So as to choose healthy and high-quality blastocysts,Transplanted into the female uterus,Ensure that the fetus is not affected by genetic diseases,Then avoid parents carrying recessive genetic genes from passing on the disease to the next generation。

therefore,United StatesHRCReproductive experts before embryo transfer,Utilizing third-generation in vitro fertilizationPGS/PGDTechnology for genetic screening and diagnosis of embryos,Then choose high-quality and healthy blastocyst transplantation,Thereby improving the clinical pregnancy rate of women,Reduce the rate of spontaneous abortion,Prevent fetal malformation、Developmental dysplasia、Problems such as congenital idiocy or genetic disorders,Realize eugenics and nurturing。